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Catalog Number | orb1946574 |
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Category | Assays and Kits |
Description | Porcine T4(Thyroxine) ELISA Kit |
Reactivity | Porcine |
Range | 4.69-300 ng/mL |
Target | T4 |
Sensitivity | 1.42 ng/mL |
Alternative names | 3,5,3',5'-Tetraiodothyronine; L-Thyroxine; Levothy Read more... |
Note | For research use only |
Application notes | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Thyroxine(T4) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Thyroxine(T4). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Thyroxine(T4) in the samples is then determined by comparing the OD of the samples to the standard curve. |
Sample Types | serum, plasma and other biological fluids |
Expiration Date | Please enquire. |
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