Porcine CRH(Corticotropin Releasing Hormone) ELISA Kit

• Catalog Number: orb1946423
• Category: Assays and Kits
• Description: Porcine CRH(Corticotropin Releasing Hormone) ELISA Kit
• Reactivity: Porcine
• Range: 0.16-10 ng/mL
• Target: CRH
• Sensitivity: 0.061 ng/mL
• Alternative names: CRF; Corticotropin Releasing Factor; Corticoliberi
• Note: For research use only
• Application notes: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Corticotropin Releasing Hormone(CRH) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Corticotropin Releasing Hormone(CRH). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 10 nm. The concentration of Corticotropin Releasing Hormone(CRH) in the samples is then determined by comparing the OD of the samples to the standard curve.
• Sample Types: serum, plasma and other biological fluids
• Expiration Date: Please enquire.