Porcine AP(Aprotinin) ELISA Kit

• Catalog Number: orb1946479
• Category: Assays and Kits
• Description: Porcine AP(Aprotinin) ELISA Kit
• Reactivity: Porcine
• Range: 1.57-100 ng/mL
• Target: AP
• Sensitivity: 0.47 ng/mL
• Alternative names: BPTI; Trasylol; Pancreatic Trypsin Inhibitor; Basi
• Note: For research use only
• Application notes: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Aprotinin(AP) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Aprotinin(AP). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Aprotinin(AP) in the samples is then determined by comparing the OD of the samples to the standard curve.
• Sample Types: Serum, plasma and other biological fluids.
• Expiration Date: Please enquire.