POMC Antibody

• Catalog Number: orb1252363
• Category: Antibodies
• Description: POMC Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: SPM501
• Tested applications: ELISA, FC, IF, IHC
• Reactivity: Human
• Isotype: IgG1
• Immunogen: Amino acids 25-39 (NGAEDESAEAFPLEF) of Adrenocorticotropic hormone (CLIP sequence) were used as the immunogen for this ACTH antibody.
• Concentration: 0.2 mg/mL
• Dilution range: ELISA: order BSA free format for coatingFlow Cytometry: 0.5-1ug/10^6 cellsIF: 1-2 ug/mlIHC (FFPE):0.5-1 ug/mlThe concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the ACTH antibody to be titered up or down for optimal performance.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: POMC
• UniProt ID: P01189
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: POMC, proopiomelanocortin, Precursor of MSH, POC,
• Note: For research use only
• Application notes: ELISA: order BSA free format for coatingFlow Cytometry: 0.5-1ug/10^6 cellsIF: 1-2 ug/mlIHC (FFPE):0.5-1 ug/mlThe concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the ACTH antibody to be titered up or down for optimal performance.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human pituitary gland with ACTH antibody (clone SPM501). Required HIER: steam sections in 10mM citrate buffer, pH6, for 10-20 min.