POLR3D Antibody

• Catalog Number: orb1526884
• Category: Antibodies
• Description: POLR3D Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human
• Immunogen: between 175 and 225
• Concentration: 200 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 10 µg/mg lysate; IHC - 1:200 - 1:1,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: POLR3D
• UniProt ID: P05423
• NCBI: NP_001713.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: polymerase (RNA) III (DNA directed) polypeptide D,
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human POLR3D by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-POLR3D antibody orb1526884 used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 10 µg/mg lysate. POLR3D was also immunoprecipitated by rabbit anti-POLR3D antibody, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 3 seconds (B).

Detection of human POLR3D by immunohistochemistry.Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-POLR3D (Cat. No. orb1526884) used at a dilution of 1: 1, 000 (0.2 µg/ml). Detection: DAB