POLR3B antibody

• Catalog Number: orb319011
• Category: Antibodies
• Description: Rabbit polyclonal antibody to POLR3B
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human, Mouse, Porcine, Primate, Rabbit, Rat, Zebrafish
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human POLR3B. The exact sequence is proprietary.
• Dilution range: WB: 1:500-1:1000, IHC-P: 1:100-1:200
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: POLR3B
• Entrez: 70428, 55703
• UniProt ID: P59470, Q9NW08
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti DNA-directed RNA polymerase III subunit RPC2
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of POLR3B expression in K562 (A), U87MG (B), HEK293T (C), CT26 (D), H9C2 (E) whole cell lysates. (Predicted band size: 127 kD; Observed band size: 128 kD)

Immunohistochemical analysis of POLR3B staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.