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Catalog Number | orb1937420 |
---|---|
Category | Antibodies |
Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Clone Number | RB31123 |
Tested applications | FC, IHC-P, WB |
Predicted Reactivity | Rat |
Reactivity | Human |
Isotype | Rabbit IgG |
Antibody Type | Primary Antibody |
Dilution range | WB: 1:1000, IHC-P: 1:10~50, FC: 1:10~50 |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Conjugation | Unconjugated |
MW | 128229 Da |
Target | This POLR1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 89-117 amino acids from the N-terminal region of human POLR1B. |
UniProt ID | Q9H9Y6 |
NCBI | NP_061887.2, NP_001131076.1 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | DNA-directed RNA polymerase I subunit RPA2, RNA po Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
POLR1B Antibody (N-term) western blot analysis in Hela cell line lysates (35 ug/lane). This demonstrates the POLR1B antibody detected the POLR1B protein (arrow).
POLR1B Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
POLR1B Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of POLR1B Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.