POLL Antibody

• Catalog Number: orb1247085
• Category: Antibodies
• Description: POLL Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-LGLPYREPAERDW
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 60kDa band observed in cell lysates of A549 and approx 60-70kDa in cell lysates of HepG2 (calculated MW of 63.5kDa according to Human NP_037406.1). Recommended concentration: 0.5-1ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: POLL
• UniProt ID: Q9UGP5
• NCBI: NP_037406.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: POLL, BETA-N, POL-KAPPA, polymerase (DNA directed)
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 60kDa band observed in cell lysates of A549 and approx 60-70kDa in cell lysates of HepG2 (calculated MW of 63.5kDa according to Human NP_037406.1). Recommended concentration: 0.5-1ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1247085 (5 ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.

orb1247085 (0.5 ug/ml) staining of A549 (A) and (1 ug/ul) HepG2 (B) cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.