PolA2 Antibody

• Catalog Number: orb1526550
• Category: Antibodies
• Description: PolA2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IP, WB
• Reactivity: Human
• Immunogen: between 448 and 498
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:1,000 - 1:5,000; IP - 5 - 15 µg/mg lysate
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: PolA2
• UniProt ID: Q14181
• NCBI: NP_002680.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: DNA polymerase alpha 70 kDa subunit; polymerase (D
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human PolA2 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-PolA2 antibody orb1526550 used for WB at 0.4 µg/ml (A) and 1 µg/ml (B) and used for IP at 10 µg/mg lysate. PolA2 was also immunoprecipitated by rabbit anti-PolA2 antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 1 minute (A) and 10 seconds (B).