PODXL antibody

• Catalog Number: orb19524
• Category: Antibodies
• Description: Goat polyclonal antibody to PODXL
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Canine, Human, Mouse, Rat
• Dilution range: ELISA: 1:8000, WB: 1-3 μg/ml
• Conjugation: Unconjugated
• MW: 58.6; 55.4
• Target: PODXL
• Entrez: 5420
• Protein Sequence: DNLTKDDLDEEEDTH
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti PODXL antibody, anti podocalyxin-like antibod
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 160kDa band observed in Human Kidney and Mouse Kidney lysates (calculated MW of 58.6kDa according to NP_001018121.1 and 55.4kDa according to NP_005388.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Kershaw et al., J Biol Chem. 1997 Jun 20;272(25):15708-14.; PMID: 9188463). Recommended concentration: 0.5-1.5 μg/ml.
• Expiration Date: 12 months from date of receipt.

orb19524 (0.5ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.