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PODXL Antibody (C-term) (Ascites)
Catalog Number: orb652178
| Catalog Number | orb652178 |
|---|---|
| Category | Antibodies |
| Description | Mouse monoclonal antibody to PODXL |
| Species/Host | Mouse |
| Clonality | Monoclonal |
| Clone Number | 632CT5.11.4 |
| Tested applications | IF, WB |
| Reactivity | Human |
| Isotype | IgG1 |
| Immunogen | Synthetic Peptide |
| Antibody Type | Primary Antibody |
| Dilution range | IF: 1:100 |
| Form/Appearance | Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide. |
| Conjugation | Unconjugated |
| MW | 58635 |
| Target | PODXL |
| UniProt ID | O00592 |
| NCBI | NP_005388.2, NP_001018121.1 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Anti-Podocalyxin antibody, anti-GCTM-2 antigen ant Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Anti-PODXL Antibody (C-term) (Ascites) at 1:16000 dilution + A549 whole cell lysate. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 58635 Da. Blocking/Dilution buffer: 5% NFDM/TBST.
Fluorescent confocal image of SY5Y cells stained with PODXL (C-term) Antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with PODXL (C-term) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min).
