Podoplanin (MAP tag) Antibody [PMab-1], Rabbit IgG - Research Grade Biosimilar

• Catalog Number: orb1671847
• Category: Antibodies
• Description: Podoplanin (MAP tag) Antibody [PMab-1], Rabbit IgG - Research Grade Biosimilar
• Clonality: Recombinant
• Clone Number: PMab-1
• Tested applications: Blocking, ELISA, Epitope Tagging, IHC, WB
• Reactivity: Mouse
• Isotype: IgG kappa
• Immunogen: This antibody was raised by immunising rats with 14-residue synthetic peptide mpp3851, which corresponds to amino acids 38-51 (GDGMVPPGIEDKIT) of the platelet-aggregation-stimulating (PLAG) domain of mouse podoplanin. Spleen cells were then harvested, and fused to P3U1 cells to generate stable hybridomas.
• Antibody Type: Primary Antibody
• Concentration: batch dependent
• Conjugation: Unconjugated
• Target: Pdpn
• UniProt ID: Q62011
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: PDPN, Aggrus, Glycoprotein 38, Gp38, OTS-8, Ots8,
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot using anti-Podoplanin (MAP tag) antibody PM536. Mouse thymus (A)- mouse brain (B)- NIH3T3 cells (C) and mouse kidney (D) lysates (35 ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric r version of 1B4 (3.0) at 0.01 ug/ml- 0.1 ug/ml- 0.001 ug/ml and 0.003 ug/ml- respectively- before detection using an anti-rondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.

Flow-cytometry using the anti-Podoplanin (MAP tag) antibody PM0875. NIH3T3 cells were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control - black line) or the r-chimeric version of PM536 (blue line) at a dilution of 1:100 for 1h at RT. After washing- bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.