pNPP 1-Component AP Microwell Substrate with Stabilizing Pellets (SUBP)

SKU: orb511046

Description

pNPP 1-Component AP Microwell Substrate with Stabilizing Pellets is a ready-to-use alkaline phosphatase substrate formulation to detect alkaline phosphatase-conjugated molecules in ELISA and microwell assays.

Images & Validation

• Application Notes:
  1. Allow product to equilibrate to room temperature (25°C) prior to use., 2. Completely cover the membrane surface with the substrate. The substrate will react with peroxidase creating an insoluble permanent dark blue reaction product., 3. To stop the reaction, rinse the developed membrane with high quality water., 4. For best results, the reaction should be monitored and read before the background staining becomes too intense to distinguish from the positive staining.

Storage & Handling

• Storage: 2-8°C
• Concentration: 1 X
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only