PNPLA3 antibody

• Catalog Number: orb19804
• Category: Antibodies
• Description: Goat polyclonal antibody to PNPLA3
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human, Mouse, Rat
• Dilution range: ELISA: 1:16000, WB: 0.01-0.03 μg/ml, IHC-P: 8 μg/ml
• Conjugation: Unconjugated
• MW: 42.6
• Target: Pnpla3 / Adiponutrin (mouse)
• Entrez: 80339
• Protein Sequence: EHDICPKVKSTN
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti Pnpla3 antibody, anti patatin-like phospholip
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.WB: Approx 45kDa band observed in Mouse Heart lysates (calculated MW of 42.6kDa according to Human NP_473429.2 and to Mouse NP_473429.2). Recommended concentration: 0.05-0.2 μg/ml.
• Expiration Date: 12 months from date of receipt.

orb19804 (0.03µg/ml) staining of Mouse Adrenal Gland lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

orb19804 Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.

orb19804 (8µg/ml) staining of paraffin embedded Human Kidney. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

orb19804 Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing Mitochondrial staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml). This data is from a previous batch, not on sale.

Immunofluorescence analysis of HepG2 cells using PNPLA3 antibody

Immunohistochemical analysis of Human Kidney using PNPLA3 antibody

Immunohistochemical analysis of Human Kidney using PNPLA3 antibody

Western blot analysis of Mouse Heart lysate using PNPLA3 antibody