PNPLA2 Antibody

• Catalog Number: orb1247752
• Category: Antibodies
• Description: PNPLA2 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine, Rat
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-NIIEVSKEARKR
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 60kDa band observed in Human Adipose lysates (calculated MW of 55.3kDa according to NP_065109.1). Recommended concentration: 0.5-2ug/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Rat Testis for this particular batch.Immunohistochemistry:In paraffin embedded Human Heart shows intense staining of distinct spots in the myocardial fibres in transverse section. Recommended concentration: 2-3ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: PNPLA2
• UniProt ID: Q96AD5
• NCBI: NP_065109.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: ATGL, DESNUTRIN, PNPLA2, patatin-like phospholipas
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 60kDa band observed in Human Adipose lysates (calculated MW of 55.3kDa according to NP_065109.1). Recommended concentration: 0.5-2ug/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Rat Testis for this particular batch.Immunohistochemistry:In paraffin embedded Human Heart shows intense staining of distinct spots in the myocardial fibres in transverse section. Recommended concentration: 2-3ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1247752 (0.5 ug/ml) staining of Human Adipose lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1247752 (2.5 ug/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.