PNCK Antibody

• Catalog Number: orb1263471
• Category: Antibodies
• Description: PNCK Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit Ig
• Immunogen: This PNCK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 314-343 amino acids from the C-terminal region of human PNCK.
• Concentration: batch dependent
• Dilution range: For IHC-P starting dilution is: 1:10~50For WB starting dilution is: 1:1000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 39 kDa
• Target: PNCK
• UniProt ID: Q6P2M8
• NCBI: Q6P2M8
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Calcium/calmodulin-dependent protein kinase type 1
• Note: For research use only
• Application notes: For IHC-P starting dilution is: 1:10~50For WB starting dilution is: 1:1000
• Expiration Date: 12 months from date of receipt.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.

Neo, HA-Pnck and HA-Pnck T171A HEK-293 cells were plated at low (LD) and high (HD) cell density and lysates prepared after 48 hours. Equal amounts of total protein were immunoblotted for HA-Pnck (WB: Pnck) expression.