PMAIP1 antibody

• Catalog Number: orb412881
• Category: Antibodies
• Description: Rabbit polyclonal antibody to PMAIP1
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: KLH-conjugated synthetic peptide of human NOXA
• Dilution range: WB: 1:500-2000
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: PMAIP1
• Entrez: 5366, 492821, 58801
• UniProt ID: Q5U777, Q13794, Q9JM54
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti-NOXA antibody, anti-Phorbol-12-myristate-13-a
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of NOXA expression in Jurkat (A), Hela (B), SKOV3 (C) whole cell lysates. (Predicted band size: 6; 14 kD; Observed band size: 14 kD)

Immunofluorescent analysis of NOXA staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).