PLCB3 (phospho-S537) antibody

• Catalog Number: orb214402
• Category: Antibodies
• Description: Rabbit polyclonal antibody to PLCB3
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S537 of human PLC beta 3 protein. The exact sequence is proprietary.
• Dilution range: WB: 1-500-1-1000
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: PLCB3
• Entrez: 5331
• UniProt ID: P51432, Q01970, Q99JE6
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti 1-phosphatidylinositol 4,5-bisphosphate phosp
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of PLC beta 3 (Phospho-S537) expression in HeLa PMA-treated (A), MCF7 UV-treated (B), mouse brain (C), rat brain (D) whole cell lysates. (Predicted band size: 138 kD; Observed band size: 150 kD)

Immunohistochemical analysis of PLC beta 3 (Phospho-S537) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.