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Catalog Number | orb769480 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to PLC gamma 1 (phospho-Tyr1253) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | The antiserum was produced against synthesized peptide derived from human PLCG1 around the phosphorylation site of Tyr1253. AA range:1221-1270 |
Concentration | 1 mg/ml |
Conjugation | Unconjugated |
MW | 150 |
Storage | Store at -20°C. Avoid repeated freeze-thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
Alternative names | Anti-PLCG1 antibody, anti-PLC1 antibody, anti-1-ph Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using PLCG1 (Phospho-Tyr1253) Antibody.
Western blot analysis of lysates from LOVO cells treated with and 293 cells treated with heat shock, using PLCG1 (Phospho-Tyr1253) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).
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