PLAU Antibody

• Catalog Number: orb1262712
• Category: Antibodies
• Description: PLAU Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit Ig
• Immunogen: This Urokinase (PLAU) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 396-426 amino acids from the C-terminal region of human Urokinase (PLAU).
• Concentration: batch dependent
• Dilution range: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:10~50For IF starting dilution is: 1:10~50For FACS starting dilution is: 1:10~50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 49 kDa
• Target: PLAU
• UniProt ID: P00749
• NCBI: P00749
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Urokinase-type plasminogen activator, U-plasminoge
• Note: For research use only
• Application notes: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:10~50For IF starting dilution is: 1:10~50For FACS starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

Western blot analysis of anti-PLAU Pab in CEM cell line lysate (35 ug/lane)

Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with PLAU antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Confocal immunofluorescent analysis of Urokinase (PLAU) Antibody with A2058 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Flow cytometric analysis of A2058 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.