PLASTIN3 Rabbit Polyclonal Antibody

• Catalog Number: orb6736
• Category: Antibodies
• Description: PLASTIN3 Rabbit Polyclonal Antibody
• Target: PLS3
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Canine, Equine, Porcine, Rabbit, Rat
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic peptide derived from human PLASTIN3 (401-500/630aa)
• UniProt ID: P13797
• RRID: AB_10927362
• MW: 69 kDa
• Tested applications: FC, IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Plastin 3(T isoform); PLS3; PLS 3; PLS-3; Plastin
• Research Area: Actin Crosslinking, Cell Biology, Signal Transduct
• Note: For research use only

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (PLASTIN3) Polyclonal Antibody, Unconjugated (orb6736) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: NIH/3T3 (Mouse) Cell Lysate at 30 ug, Primary: Anti-PLASTIN3 (orb6736) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 69 kD, Observed band size: 69 kD.