PKN1 (phospho-T774/816) antibody

• Catalog Number: orb515530
• Category: Antibodies
• Description: Rabbit polyclonal antibody to PKN1 (phospho-T774/816)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Reactivity: Human, Mouse, Rat
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T774/816 of human PKN1/2 protein. The exact sequence is proprietary.
• Dilution range: WB: 1:500-1000
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: PKN1; PKN2
• Entrez: 5586, 29355, 320795, 5585, 109333
• UniProt ID: Q8BWW9, Q16512, Q63433, P70268, Q16513, O08874
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: PKN1; PAK1; PKN; PRK1; PRKCL1; Serine/threonine-pr
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of PKN1/2 (Phospho-T774/816) expression in HCT116 (A) whole cell lysates. (Predicted band size: 103; 112 kD; Observed band size: 130 kD)

Direct ELISA antibody dose-response curve using Anti-PKN1/2 (Phospho-T774/816) Antibody. Antigen (Phosphopeptide and non-phosphopeptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.