PKM2 (N-term E131) Antibody

• Catalog Number: orb1928125
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB15338
• Tested applications: FC, IHC-P, WB
• Predicted Reactivity: Mouse, Rabbit, Rat
• Reactivity: Human
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:1000, IHC-P: 1:50~100, FC: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 57937 Da
• Target: This PKM2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 116-145 amino acids from the N-terminal region of human PKM2.
• UniProt ID: P14618
• NCBI: NP_001193728.1, NP_872270.1, NP_001193727.1, NP_872271.1, NP_002645.3, NP_001193725.1, NP_001193726.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Pyruvate kinase PKM, Cytosolic thyroid hormone-bin
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of PKM2 (arrow) using rabbit polyclonal PKM2 Antibody (N-term E131). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PKM2 gene (Lane 2).

PKM2 (N-term E131) Antibody flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

PKM2 (N-term E131) Antibody IHC analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PKM2 (N-term E131) Antibody for immunohistochemistry. Clinical relevance has not been evaluated.