PKM2 specific Mouse Monoclonal Antibody

SKU: orb395451

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Description

Mouse monoclonal antibody to PKM2-specific.

Research Area

Epigenetics

Images & Validation

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Item 1 of 4

Tested Applications	ELISA, FC, IF, IHC, IP, WB
Dilution Range	WB: 1:2000-1:16000; IP: 1:200-1:1000; IHC: 1:100-1:1000; IF: 1:20-1:200
Reactivity	Human, Mouse, Rat

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	IgG1
Immunogen	PKM2 pyruvate kinase, muscle
Target	PKM2
Purity	≥95% as determined by SDS-PAGE
Purification	Protein A+G purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	liquid
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Anti-CTHBP antibody, anti-HEL-S-30 antibody, anti-OIP3 antibody, anti-PK3 antibody, anti-PKM2 antibody, anti-TCB antibody, anti-THBP1 antibody

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Hela cells were subjected to SDS PAGE followed by western blot with orb395451 (PKM2-specific antibody) at dilution of 1:1000.

Immunofluorescent analysis of HepG2 cells using orb395451 (PKM2-specific antibody) at dilution of 1:50 and Rhodamine-labeled goat anti-mouse IgG (red).

IP Result of anti-PKM2-specific (IP: orb395451, 4 ug; Detection: orb395451 1:300) with HeLa cells lysate 2400 ug.

Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using orb395451 (PKM2-specific Antibody) at dilution of 1:50.