PKC theta (phospho-S695) antibody

• Catalog Number: orb338830
• Category: Antibodies
• Description: Rabbit polyclonal antibody to PRKCQ
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S695 of human PKC theta protein. The exact sequence is proprietary.
• Dilution range: WB: 1:500-1000, IHC-P: 1:100-200
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: PRKCQ
• Entrez: 5588
• UniProt ID: Q04759
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti PRKCT antibody, anti Protein kinase C theta t
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of PKC theta (Phospho-S695) expression in Jurkat PMA-treated (A), HEK293T PMA-treated (B) whole cell lysates. (Predicted band size: 81 kD; Observed band size: 80 kD)

Immunohistochemical analysis of PKC theta (Phospho-S695) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.