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Catalog Number | orb234367 |
---|---|
Category | Antibodies |
Description | PKC iota/PRKCI Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | Rabbit IgG |
Immunogen | E.coli-derived human PKC iota recombinant protein (Position: D21-Q214). Human PKC iota shares 96% and 97% amino acid (aa) sequence identity with mouse and rat PKC iota, respectively. |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Dilution range | Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By HeatWestern blot, 0.1-0.5μg/ml, Human, Mouse, Rat |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 68262 MW |
UniProt ID | P41743 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | Protein kinase C iota type;2.7.11.13;Atypical prot Read more... |
Note | For research use only |
Application notes | WB: The detection limit for PKC iota is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
IHC(P) analysis of Mouse Brain Tissue using Anti-PKC iota Picoband antibody.
IHC(P) analysis of Rat Brain Tissue using Anti-PKC iota Picoband antibody.
IHC(P) analysis of Human Lung Cancer Tissue using Anti-PKC iota Picoband antibody.
Western blot analysis using Anti-PKC iota Picoband antibody.Lane 1:SHG Cell;2:A549 Cell;3:U87 Cell;4:293T Cell;5:HELA Cell;6:JURKAT Cell.
IHC-P | |
Canine, Feline, Human | |
Mouse | |
Monoclonal | |
Unconjugated |
FC, IHC-P, WB | |
Bovine, Equine, Porcine, Rat, Sheep |
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