PKC epsilon/PRKCE Antibody

• Catalog Number: orb234366
• Category: Antibodies
• Description: PKC epsilon/PRKCE Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Predicted Reactivity: Bovine, Canine, Equine, Monkey
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human PKC epsilon recombinant protein (Position: Q53-R236). Human PKC epsilon shares 99% amino acid (aa) sequence identity with both mouse and rat PKC epsilon.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, Human, By HeatWestern blot, 0.1-0.5μg/ml, Human, Mouse, Rat
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 83674 MW
• UniProt ID: Q02156
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Protein kinase C epsilon type;2.7.11.13;nPKC-epsil
• Note: For research use only
• Application notes: WB: The detection limit for PKC epsilon is approximately 0.1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

IHC(P) analysis of Mouse Intestine Tissue using Anti-PKC epsilon Picoband antibody.

IHC(P) analysis of Rat Intestine Tissue using Anti-PKC epsilon Picoband antibody.

Western blot analysis using Anti-PKC epsilon Picoband antibody.Lane 1:Rat Brain Tissue;2:Mosue Brain Tissue;3:MCF-7 Cell.