PKC epsilon (Phospho-S729) antibody

• Catalog Number: orb315664
• Category: Antibodies
• Description: Rabbit polyclonal antibody to PKC epsilon (Phospho-S729)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Rabbit, Rat
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S729 of human PKC epsilon protein. The exact sequence is proprietary.
• Dilution range: WB: 1-500:1000, IF/ICC: 1-100:500
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: PRKCE
• Entrez: 29340, 5581, 18754
• UniProt ID: Q02156, P09216, P16054
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti PKCE antibody, anti Protein kinase C epsilon
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Di Pietro, Paola et al. Targeting the ASMase/S1P pathway protects from sortilin-evoked vascular damage in hypertension J Clin Invest, 132, e146343 (2022)

PubMed: 35104805

Western blot analysis of PKC epsilon (Phospho-S729) expression in HEK293T (A), H1792 (B) whole cell lysates. (Predicted band size: 83 kD; Observed band size: 82 kD)

Immunofluorescent analysis of PKC epsilon (Phospho-S729) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).