PKC delta/PRKCD Antibody

• Catalog Number: orb234285
• Category: Antibodies
• Description: PKC delta/PRKCD Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IF, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human PKC delta recombinant protein (Position: M1-E160). Human PKC delta shares 95% and 93% amino acid (aa) sequence identity with mouse and rat PKC delta, respctively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 77505 MW
• UniProt ID: Q05655
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Protein kinase C delta type;2.7.11.13;Tyrosine-pro
• Note: For research use only
• Application notes: WB: The detection limit for PKC delta is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

WB analysis using Anti-PKC delta antibody.Lane 1: HELA Cell; 2:SMMC Cell;3: U87 Cell;4: JURKAT Cell;5: CEM Cell;6: A549 Cell.

IF analysis of PKC delta using anti-PKC delta antibody. PKC delta was detected in immunocytochemical section of A549 cells.