PIWIL3 Antibody

• Catalog Number: orb1239219
• Category: Antibodies
• Description: PIWIL3 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: PIWI-L3 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human PIWI-L3.The immunogen is located within amino acids 130 - 180 of PIWI-L3.
• Concentration: 1 mg/mL
• Dilution range: PIWI-L3 antibody can be used for detection of PIWI-L3 by Western blot at 1 μg/mL. Antibody can also be used for immunofluorescence starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: PIWIL3
• UniProt ID: Q7Z3Z3
• NCBI: NP_001008496
• Storage: PIWI-L3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: PIWI-L3 Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: PIWI-L3 Antibody: HIWI3, Piwi-like protein 3
• Note: For research use only
• Application notes: PIWI-L3 antibody can be used for detection of PIWI-L3 by Western blot at 1 μg/mL. Antibody can also be used for immunofluorescence starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of PIWI-L3 in 3T3 cell lysate with PIWI-L3 antibody at 1 µg/mL in (A) the absence and (B) the presence of blocking peptide.

Immunofluorescence of PIWI-L3 in 3T3 cells with PIWI-L3 antibody at 20 µg/mL.