PIWIL1 Antibody

• Catalog Number: orb1239221
• Category: Antibodies
• Description: PIWIL1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Predicted Reactivity: Gallus
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: PIWI-L1 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human PIWI-L1.The immunogen is located within amino acids 60 - 110 of PIWI-L1.
• Concentration: 1 mg/mL
• Dilution range: PIWI-L1 antibody can be used for detection of PIWI-L1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 85, 90 kDa Observed: 95 kDa
• Target: PIWIL1
• UniProt ID: Q96J94
• NCBI: AAK69348
• Storage: PIWI-L1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: PIWI-L1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: PIWI-L1 Antibody: HIWI, MIWI, PIWI, CT80.1, HIWI,
• Note: For research use only
• Application notes: PIWI-L1 antibody can be used for detection of PIWI-L1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of PIWI-L1 in human testis tissue lysate with PIWI-L2 antibody at 1 µg/ml in (A) the absence and (B) the presence of blocking peptide.

Immunohistochemistry of PIWI-L1 in rat liver tissue with PIWI-L1 antibody at 10 µg/mL.

Immunofluorescence of PIWI-L1 in rat liver tissue with PIWI-L1 antibody at 20 µg/mL.