PITX2 Antibody

• Catalog Number: orb1268408
• Category: Antibodies
• Description: PITX2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Predicted Reactivity: Gallus, Mouse, Rat, Xenopus, Zebrafish
• Reactivity: Human
• Isotype: Rabbit Ig
• Immunogen: This PITX2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 122-151 amino acids of human PITX2.
• Concentration: batch dependent
• Dilution range: For IF starting dilution is: 1:10~50For WB starting dilution is: 1:1000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 35 kDa
• Target: PITX2
• UniProt ID: Q99697
• NCBI: Q99697
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Pituitary homeobox 2, ALL1-responsive protein ARP1
• Note: For research use only
• Application notes: For IF starting dilution is: 1:10~50For WB starting dilution is: 1:1000
• Expiration Date: 12 months from date of receipt.

Fluorescent image of Hela cell stained with PITX2 Antibody. Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with PITX2 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7 units/ml). PITX2 immunoreactivity is localized to Nucleus significantly.

Western blot analysis of anti-Pilx2 Pab in CEM cell line lysates (35 ug/lane).