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Catalog Number | orb1929349 |
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Category | Antibodies |
Description | Purified Rabbit Polyclonal Antibody (Pab) |
Target | This PITX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 123-150 amino acids from the Central region of human PITX1. |
Clonality | Polyclonal |
Species/Host | Rabbit |
Isotype | Rabbit IgG |
Conjugation | Unconjugated |
Reactivity | Human |
Predicted Reactivity | C. elegans, Drosophila, Mouse, Other, Rat, Zebrafish |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
UniProt ID | P78337 |
MW | 34128 Da |
Tested applications | FC, IHC-P, WB |
Dilution range | WB: 1:1000, WB: 1:1000, IHC-P: 1:50~100, FC: 1:10~50 |
Antibody Type | Primary Antibody |
Clone Number | RB18913 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | Pituitary homeobox 1, Hindlimb-expressed homeobox Read more... |
Note | For research use only |
NCBI | NP_002644.4 |
PITX1 Antibody (Center) flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
PITX1 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded human Lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PITX1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Western blot analysis of PITX1 antibody (Center) in NCI-H460 cell line lysates (35 ug/lane). PITX1 (arrow) was detected using the purified Pab.
Western blot analysis of PITX1 (arrow) using rabbit polyclonal PITX1 Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PITX1 gene.