PIR Antibody

• Catalog Number: orb1247097
• Category: Antibodies
• Description: PIR Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Mouse
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: GSSKKVTLSVLSREC
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 33kDa band observed in Human Heart lysate (calculated MW of 32.1kDa according to NP_003653.1). Recommended concentration: 0.3 - 1.0 ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Liver and Spleen. Recommended concentration: 5ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: PIR
• UniProt ID: O00625
• NCBI: NP_003653.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: PIR, pirin, pirin (iron-binding nuclear protein)
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 33kDa band observed in Human Heart lysate (calculated MW of 32.1kDa according to NP_003653.1). Recommended concentration: 0.3 - 1.0 ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Liver and Spleen. Recommended concentration: 5ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1247097 (0.3 ug/ml) staining of Human Heart lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1247097 (5 ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1247097 (5 ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.