PIPES

SKU: orb1690455

Description

PIPES (1,4-Piperazinediethanesulfonic acid) is a zwitterionic buffering agent essential for maintaining physiological pH in biochemical research. It is widely employed in in vitro cell culture, protein studies, and various molecular biology applications due to its minimal interference with biological processes.

Key Properties

• CAS Number: 5625-37-6
• MW: 302.37
• Purity: 99.00% (May vary between batches)
• Formula: C₈H₁₈N₂O₆S₂
• SMILES: OS(=O)(=O)CCN1CCN(CCS(O)(=O)=O)CC1
• Target: Others
• Solubility: DMSO:< 1 mg/mL (insoluble or slightly soluble);H2O:< 1 mg/mL (insoluble)

Bioactivity

• In Vitro:
  To prepare the PIPES buffer, dissolve 173 g of 1,4-piperazinediethanesulfonic acid in 1 L of deionized water. Adjust the pH of the solution to 6.8 using sodium hydroxide pellets. This buffer is efficacious in mitigating lipid loss and artifacts induced by glutaraldehyde fixation .

Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

1,4-piperazine bis(ethanesulfonic acid), 1,4-Piperazinebis(ethanesulfonic acid), 1,4-Piperazinediethanesulfonic acid, PIPES

Compound Identifiers

PubChem CID

79723