PIP5K1-beta antibody

• Catalog Number: orb667703
• Category: Antibodies
• Description: Rabbit polyclonal antibody to PIP5K1-beta
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant fusion protein of human PIP5K1-beta. The exact sequence is proprietary.
• Dilution range: WB: 1-500-2000, IF: 1-50-200
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: PIP5K1B
• Entrez: 18719, 309419, 8395
• UniProt ID: O14986, Q5CZZ9, P70181
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: Anti-STM7 antibody, anti-Phosphatidylinositol 4-ph
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of PIP5K1-beta expression in mouse lung (A) whole cell lysates. (Predicted band size: 56; 61; 62 kD; Observed band size: 71 kD)

Immunofluorescent analysis of PIP5K1-beta staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 488-conjugated secondary antibody (green) in PBS at room temperature in the dark.