PIK3R3 Antibody

• Catalog Number: orb1262799
• Category: Antibodies
• Description: PIK3R3 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit Ig
• Immunogen: This PIK3R3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 316-346 amino acids from the C-terminal region of human PIK3R3.
• Concentration: batch dependent
• Dilution range: For IF starting dilution is: 1:100For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:10~50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 54 kDa
• Target: PIK3R3
• UniProt ID: Q92569
• NCBI: Q92569
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Phosphatidylinositol 3-kinase regulatory subunit g
• Note: For research use only
• Application notes: For IF starting dilution is: 1:100For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

Fluorescent image of U251 cells stained with PIK3R3 Antibody. Antibody was diluted at 1:100 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).

Western blot analysis of lysate from MDA-MB-453 cell line, using PI3KR3 Antibody (G331) RB1719 was diluted at 1:1000 at each lane.

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PIK3R3 antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.