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Catalog Number | orb1088226 |
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Category | Assays and Kits |
Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Procollagen III N-Terminal Propeptide(PIIINP). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Procollagen III N-Terminal Propeptide(PIIINP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Procollagen III N-Terminal Propeptide(PIIINP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Procollagen III N-Terminal Propeptide(PIIINP) in the samples is then determined by comparing the OD of the samples to the standard curve. |
Reactivity | Porcine |
Range | 6.25-400 ng/mL |
Sensitivity | 2.33 ng/mL |
Protein Length | 3.5h |
Alternative names | P3NP, N-Propeptide Of Type III Procollagen, Procol Read more... |
Note | For research use only |
Sample Types | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
Expiration Date | Please enquire. |
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