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Catalog Number | orb1088232 |
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Category | Assays and Kits |
Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Anti-Smooth Muscle Antibody(ASMA). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Anti-Smooth Muscle Antibody(ASMA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Anti-Smooth Muscle Antibody(ASMA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Anti-Smooth Muscle Antibody(ASMA) in the samples is then determined by comparing the OD of the samples to the standard curve. |
Reactivity | Porcine |
Range | 7.82-500 ng/mL |
Sensitivity | 2.9 ng/mL |
Protein Length | 3.5h |
Alternative names | ASM Ab, Anti Smooth Muscle Antibody Read more... |
Note | For research use only |
Sample Types | tissue homogenates, cell lysates and other biological fluids |
Expiration Date | Please enquire. |
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