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    PICALM Antibody

    Catalog Number: orb623882

    DispatchUsually dispatched within 5-10 working days
    $ 520.00
    Catalog Numberorb623882
    CategoryAntibodies
    DescriptionPICALM Antibody
    Species/HostRabbit
    ClonalityPolyclonal
    Tested applicationsELISA, FC, ICC, IF, IHC, WB
    ReactivityHuman, Mouse, Rat
    IsotypeRabbit IgG
    ImmunogenE.coli-derived human PICALM recombinant protein (Position: M1-M652).
    ConcentrationAdding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
    Dilution rangeWestern blot, 0.25-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human Direct ELISA, 0.1-0.5μg/ml, Human
    Form/AppearanceLyophilized
    ConjugationUnconjugated
    MW71 kDa
    UniProt IDQ13492
    StorageStore at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
    Alternative namesPhosphatidylinositol-binding clathrin assembly pro
    Read more...
    NoteFor research use only
    Application notesTested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
    Expiration Date12 months from date of receipt.
    PICALM Antibody

    Western blot analysis of PICALM using anti-PICALM antibody (orb623882). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: rat lung tissue lysates, Lane 3: mouse lung tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PICALM antigen affinity purified polyclonal antibody (Catalog # orb623882) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # orb90503) with Tanon 5200 system. A specific band was detected for PICALM at approximately 71KD. The expected band size for PICALM is at 71KD.

    PICALM Antibody

    IHC analysis of PICALM using anti-PICALM antibody (orb623882). PICALM was detected in paraffin-embedded section of human Ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PICALM Antibody (orb623882) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # orb90444) with DAB as the chromogen.

    PICALM Antibody

    IF analysis of PICALM using anti-PICALM antibody (orb623882). PICALM was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (orb90553) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-PICALM Antibody (orb623882) overnight at 4°C. DyLight®488 conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

    PICALM Antibody

    Flow Cytometry analysis of A431 cells using anti-PICALM antibody (orb623882). Overlay histogram showing A431 cells stained with orb623882 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PICALM Antibody (orb623882, 1μg/1x10^6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x10^6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

    • PICALM antibody [orb418135]

      ELISA,  IF,  IHC

      Human

      Rabbit

      Polyclonal

      Unconjugated

      100 μg, 50 μg
    • PICALM Antibody [orb1239232]

      ELISA,  IF,  WB

      Human, Mouse, Rat

      Rabbit

      Polyclonal

      Unconjugated

      0.1 mg, 0.02 mg
    • Human PICALM ELISA Kit [orb778307]

      Human

      0.32-20 ng/mL

      0.118 ng/mL

      48 Test, 96 Test, 24 t
    • Rat PICALM ELISA Kit [orb782467]

      Rat

      0.32-20 ng/mL

      0.103 ng/mL

      48 Test, 96 Test, 24 t
    • PICALM antibody (HRP) [orb418136]

      ELISA

      Human

      Rabbit

      Polyclonal

      HRP

      50 μg, 100 μg
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