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Catalog Number | orb766079 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to PIAS 3 |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, IHC-P, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | The antiserum was produced against synthesized peptide derived from human PIAS3. AA range:10-59 |
Concentration | 1 mg/ml |
Conjugation | Unconjugated |
MW | 68 |
Storage | Store at -20°C. Avoid repeated freeze-thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
Alternative names | Anti-PIAS3 antibody, anti-E3 SUMO-protein ligase P Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of lysates from 1) 293, 2) Rat kidney, 3) HELA cells, (Green) primary antibody was diluted at 1:1000, 4°C over night, secondary antibody was diluted at 1:10000, 37°C 1 hour. (Red) GAPDH Monoclonal Antibody (2B8) antibody was diluted at 1:5000 as loading control, 4°C over night, secondary antibody was diluted at 1:10000, 37°C 1 hour.
Western Blot analysis of various cells using PIAS 3 Polyclonal Antibody diluted at 1:2000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit.
Western Blot analysis of 293 cells using PIAS 3 Polyclonal Antibody diluted at 1:2000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit.
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using PIAS3 Antibody. The picture on the right is blocked with the synthesized peptide.
ELISA, WB | |
Human, Monkey, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, WB | |
Human, Monkey, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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