PI3K p85 beta antibody

• Catalog Number: orb334784
• Category: Antibodies
• Description: Rabbit polyclonal antibody to PI3K p85 beta
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human
• Immunogen: Recombinant protein corresponding to human PI3K p85 beta.
• Dilution range: WB: 1:1000-1:2000, IHC-P: 1:50-1:200
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: PIK3R2
• Entrez: 5296
• UniProt ID: O00459
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti NR1C3 antibody, anti Peroxisome proliferator-
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of PI3K p85 beta expression in HepG2 (A) whole cell lysates. (Predicted band size: 81 kD; Observed band size: 82 kD)

Immunohistochemical analysis of PI3K p85 beta staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.