PI3K p85 alpha (phospho-Y607) antibody

• Catalog Number: orb338965
• Category: Antibodies
• Description: Rabbit polyclonal antibody to PIK3R1
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y607 of human PI3K p85 alpha protein. The exact sequence is proprietary.
• Dilution range: WB: 1:500-1000, IHC-P: 1:100-200
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: PIK3R1
• Entrez: 5295, 25513, 18708
• UniProt ID: P27986, Q63787, P26450
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti GRB1 antibody, anti Phosphatidylinositol 3-ki
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Tao, Haitao et al. Downregulation of miR-34a Promotes Proliferation and Inhibits Apoptosis of Rat Osteoarthritic Cartilage Cells by Activating PI3K/Akt Pathway Clin Interv Aging, 15, 373-385 (2020)

PubMed: 32214804

Applications

WB

Reactivity

Rat

Wang, Weiwei et al. Notoginsenoside R1 alleviates high glucose-evoked damage in RSC96 cells through down-regulation of miR-503 Artif Cells Nanomed Biotechnol, 47, 3947-3954 (2019)

PubMed: 31581849

Applications

WB

Reactivity

Rat

Xu, Dongsheng et al. miR-10a overexpression aggravates renal ischemia-reperfusion injury associated with decreased PIK3CA expression BMC Nephrol, 21, 248 (2020)

PubMed: 32611384

Applications

WB

Reactivity

Rat

Western blot analysis of PI3K p85 alpha (Phospho-Y607) expression in Jurkat (A), rat kidney (B) whole cell lysates. (Predicted band size: 83 kD; Observed band size: 95 kD)

Immunohistochemical analysis of PI3K p85 alpha (Phospho-Y607) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.