PI31 antibody

• Catalog Number: orb18428
• Category: Antibodies
• Description: Goat polyclonal to PI31
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IF, WB
• Reactivity: Bovine, Canine, Human, Mouse, Rat
• Dilution range: ELISA: 1:32000, WB: 0.3-1μg/ml, IF/ICC: 10μg/ml
• Conjugation: Unconjugated
• MW: 29.8
• Target: PI31 / PSMF1 (Isoform 1)
• Entrez: 9491
• Protein Sequence: DHLPPPGYDDMYL
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti PSMF1 antibody, anti PI31 antibody, anti prot
• Note: For research use only
• Application notes: This antibody is expected to recognize isoform 1 (NP_006805.2 and NP_848693.2).ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 35kDa band observed in Human Kidney lysates (calculated MW of 29.8kDa according to NP_006805.2). In transfected HEK293 transiently expressing PSMF1 a band of approx. 35kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 1-3 μg/ml.
• Expiration Date: 12 months from date of receipt.

Immunofluorescence analysis of paraformaldehyde fixed A431 cells using PI31 antibody.

Western blot analysis of KNRK (A), NIH3T3 (B and (0.3ug/ml) Neuro2a (C) cell lysate using PI31 antibody.

Western blot analysis of HEK293 (A), K562 (B) and (1ug/ml) LNCaP (C) cell lysate using PI31 antibody.