PI 3-kinase p85 alpha/gamma antibody

• Catalog Number: orb766075
• Category: Antibodies
• Description: Rabbit polyclonal antibody to PI 3-kinase p85 alpha/gamma
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Reactivity: Gallus, Human, Monkey, Mouse, Rat
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human PI3-kinase p85-alpha/gamma. AA range:436-485
• Concentration: 1 mg/ml
• Conjugation: Unconjugated
• MW: 54+83
• Storage: Store at -20°C. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Alternative names: Anti-PIK3R1 antibody, anti-GRB1 antibody, anti-Pho
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence analysis of human-lung tissue. 1, PI 3-kinase p85α/γ Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A + B.

Immunofluorescence analysis of rat-lung tissue. 1, PI 3-kinase p85α/γ Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A + B.

Western Blot analysis of various cells using primary antibody diluted at 1:1000 (4°C overnight). Secondary antibody:Goat Anti-rabbit IgG IRDye 800 ( diluted at 1:5000, 25°C, 1 hour) .

Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1, PI 3-kinase p85α/γ Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.