Phospholipase D2 (phospho-Y169) antibody

• Catalog Number: orb214409
• Category: Antibodies
• Description: Rabbit polyclonal antibody to PLD2
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human, Mouse, Primate, Rat
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y169 of human Phospholipase D2 protein. The exact sequence is proprietary.
• Dilution range: WB: 1-500-1-1000
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: PLD2
• Entrez: 18806, 25097, 5338
• UniProt ID: P70498, P97813, O14939
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti Phospholipase D2 antibody, anti PLD 2 antibod
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Phospholipase D2 (Phospho-Y169) expression in HEK293T TNFa-treated (A), NIH3T3 TNFa-treated (B), H9C2 TNFa-treated (C) whole cell lysates. (Predicted band size: 105 kD; Observed band size: 105 kD)

Immunohistochemical analysis of Phospholipase D2 (Phospho-Y169) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.