Phospho-Src (Tyr530) Antibody

• Catalog Number: orb1733007
• Category: Antibodies
• Description: Src activity is regulated by tyrosine phosphorylation at two sites, but with opposing effects. While phosphorylation at Tyr416 in the activation loop of the kinase domain upregulates enzyme activity, phosphorylation at Tyr527 in the carboxy-terminal tail by Csk renders the enzyme less active (2).
• Species/Host: Rabbit
• Clonality: Monoclonal
• Tested applications: IP, WB
• Reactivity: Rat
• Isotype: Rabbit IgG
• Immunogen: A synthetic phosphopeptide corresponding to residues surrounding Tyr529 of human Src
• Concentration: 1mg/ml
• Dilution range: IP: 1/20; WB: 1/500-1/1000
• Conjugation: Unconjugated
• MW: Calculated MW: 60 kDa; Observed MW: 60 kDa
• Entrez: 6714
• UniProt ID: P12931
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Buffer/Preservatives: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% rAlbumin
• Alternative names: SRC; SRC1; Proto-oncogene tyrosine-protein kinase
• Note: For research use only
• Expiration Date: 12 months from date of receipt.