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Catalog Number | orb548579 |
---|---|
Category | Antibodies |
Description | Phospho-Smad2 (S250) Rabbit Monoclonal Antibody |
Species/Host | Rabbit |
Clonality | Monoclonal |
Clone Number | HBC-19 |
Tested applications | FC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | Rabbit IgG |
Immunogen | A synthesized peptide derived from human Smad2 |
Concentration | Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure. |
Dilution range | WB 1:1000-1:2000FC 1:20 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | 52306 MW |
UniProt ID | Q15796 |
Storage | Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles. |
Alternative names | Mothers against decapentaplegic homolog 2;MAD homo Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of Phospho-Smad2 (S250) expression in (1) HeLa cell treated with alkaline phosphatase lysate; (2) HeLa cell lysate (orb548579). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMAD2 monoclonal antibody (Catalog # orb548579) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # orb90503) with Tanon 5200 system. A specific band was detected for SMAD2.
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