Phospho-S6K1 (T421 + S424) RPS6KB1 Rabbit Monoclonal Antibody

• Catalog Number: orb548638
• Category: Antibodies
• Description: Phospho-S6K1 (T421 + S424) RPS6KB1 Rabbit Monoclonal Antibody
• Species/Host: Rabbit
• Clonality: Monoclonal
• Clone Number: BHD-18
• Tested applications: IP, WB
• Reactivity: Human, Rat
• Isotype: Rabbit IgG
• Immunogen: A synthesized peptide derived from human Phospho-S6K1 (T421 + S424)
• Concentration: Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
• Dilution range: WB 1:500-1:2000IP 1:30
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 59140 MW
• UniProt ID: P23443
• Storage: Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
• Alternative names: Ribosomal protein S6 kinase beta-1;S6K-beta-1;S6K1
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of SK61 phosphorylation expression in HEK293 cell lysate (orb548638). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPS6KB1 monoclonal antibody (Catalog # orb548638) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # orb90503) with Tanon 5200 system. A specific band was detected for RPS6KB1.