Phospho-Nucleophosmin (Thr199) Antibody

• Catalog Number: orb1733022
• Category: Antibodies
• Description: Involved in diverse cellular processes such as ribosome biogenesis, centrosome duplication, protein chaperoning, histone assembly, cell proliferation, and regulation of tumor suppressors p53/TP53 and ARF. Binds ribosome presumably to drive ribosome nuclear export. Associated with nucleolar ribonucleoprotein structures and bind single-stranded nucleic acids. Acts as a chaperonin for the core histones H3, H2B and H4. Stimulates APEX1 endonuclease activity on apurinic/apyrimidinic (AP) double-stranded DNA but inhibits APEX1 endonuclease activity on AP single-stranded RNA. May exert a control of APEX1 endonuclease activity within nucleoli devoted to repair AP on rDNA and the removal of oxidized rRNA molecules. In concert with BRCA2, regulates centrosome duplication. Regulates centriole duplication: phosphorylation by PLK2 is able to trigger centriole replication. Negatively regulates the activation of EIF2AK2/PKR and suppresses apoptosis through inhibition of EIF2AK2/PKR autophosphorylation. Antagonizes the inhibitory effect of ATF5 on cell proliferation and relieves ATF5-induced G2/M blockade (PubMed:22528486). In complex with MYC enhances the transcription of MYC target genes (PubMed:25956029).
• Species/Host: Rabbit
• Clonality: Monoclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human, Rat
• Isotype: Rabbit IgG
• Immunogen: A synthetic phosphopeptide corresponding to residues surrounding Thr199 of human Nucleophosmin
• Concentration: 1mg/ml
• Dilution range: IP: 1/20; WB: 1/500-1/1000; IHC: 1/50-1/100
• Conjugation: Unconjugated
• MW: Calculated MW: 33 kDa; Observed MW: 38 kDa
• Entrez: 4869
• UniProt ID: P06748
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Buffer/Preservatives: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% rAlbumin
• Alternative names: B23; NPM
• Note: For research use only
• Expiration Date: 12 months from date of receipt.